Sodium Alginate and Polymer Drug Delivery Systems

Sodium Alginate and Polymer Drug Delivery SystemsSodium alginate is a hygroscopic temporal, although, unchangeable at low humidities and at cool temperatures. Aqueous solutions of sodium alginate atomic number 18 just about stable at ph 4-10. Below ph3, alginic tart is precipitated. Sodium alginate solutions atomic number 18 susceptible to microbial spoilage during storage, which may effect on solution viscosity. Subsequent loss of viscosity due to depolarization was observed when sodium alginate was heat above 70c. Preparations containing sodium alginate for external use may be preserved by the addition of 0. 1% chlorocresol, chloroxylenol, or parabens and if the medium is sexually transmitted diseaseic, benzoic acid may be utilize. Bulk material should be stored in an airtight container in a cool and dry place.Sodium alginate is incompatible with acridine derivatives, crystal violet, phenyl mercuric acetate and nitrate, thick metals and ethanol in concentrations greater than 5%w/v. Low concentrations of electrolytes cause an increase in viscosity but high electrolyte concentrations make salting out of sodium alginate salting out occurs if more than 4% of sodium chloride is present.Sodium alginate is utilize in variety of oral and pharmaceutical rowulations. In tablet peeulations, sodium alginate may be utilise as both a binder and disintegrant. It has also been used as a diluents in abridgement earnulations and also been used in the preparation of sustained lighting oral formulations, since it privy delay the dissolution of a medicate from tablets, capsules and aqueous suspensions.Recently, sodium alginate has been used for the aqueous microencapsulation of do drugss in contrast with the more conventional microencapsulation techniques which use perfect solvent establishments. It has also been used in the formation of nanoparticles.The adhesive nature of hydro gelatins inclined(p) from sodium alginate has been investigated and the drug release from oral mucosal adhesive tablets based in sodium alginate has been reported. Hydrogel musical arrangements containing alginates have also been investigated for pitch of proteins and peptides.Therapeutically sodium alginate has been used in the combination with an h2 receptor antagonist in the management of gastroesophageal reflux and as a haemostatic agent in surgical dressings. Alginate dressings, used to brood exuding wounds often contain significant amounts of sodium alginate as this improves the gelling properties. Sodium alginate is also used in cosmetics and food products at concentrations given in table 4 synthetic rubberSodium alginate is widely used in cosmetics, food products, and pharmaceutical formulations, such as topical products, including wound dressings. It is ecumenically regarded as a nonvenomous and non- sticker material, although excessive oral consumption may be harmful. The WHO has not specified an acceptable daily intake for alginic acid and al ginate salts as the levels used in foods do not represent a hazard to health.Handling precautions. Sodium alginate may be irritant to eye or respiratory system if inhaled as break upeye protection, gloves, dust respirator be needed while handling. Sodium alginate should be handled in a well ventilated environment.Related substancesThe various substances connect to sodium alginate include alginic acid, potassium alginate, calcium alginate, propylene glycol alginate.CHITOSANChitosan is a derivative of chitin and it is a unique polysaccharide and hydrophilic polymer.Non Proprietary arrive atsBP Chitosan hydrochloridePh Eur Chitosan hydrochloridumChemistryPreparationThe principle derivative of chitin, namely Chitosan (C6H11O4N)n is a unique polysaccharide and hydrophilic polymer which is taken from the chitin, a polysaccharide found in exoskeletons of crustaceans. it is treat by removing the shells from shellfish such as shrimp, lobusters and crabs. The shells are then ground int o a pulverous powder. This powder is then deacetylated. This involves boiling chitin in concentrated alkali (50%) for several(prenominal) hours. This will yield chitosan with a degree of acetylation between 20-30%, the most popular commercial form of Chitosan. In such a chitosan, the acetyl bases are uniformly distributed along the polymer chain. This is in contrast with the Chitosan of similar degree of acetylation, but isolated from fungal cell walls in which the acetylresidues are grouped into clusters. Special chemical treatments are required to obtain al atomic number 53 de-acetylated forms of chitosan.CHITINFunctional categoryIt is used as a coating agent disintegrant film forming agent mucoadhesive, tablet binder viscosity increasing agent etc.Chemical characterChitosan is a cationic polyamine with a high charge minginess at phThe amino group group in chitosan has a pka value of approximately 6. 5, thus chitosan is positively charged and soluble in acidic to unbiassed sol ution with a charge density depend on ph and the %da. Numerous studies have demonstrated that the salt form, molecular weight, and degree of deacetylation as well as ph at which chitosan is used all influence how this polymer is utilized in pharmaceutical application. Chitosan is incompatible with strong oxidising agent.Typical propertiesChitosan is a cationic polyamine with a high charge density at phAcidity / alkalinitypH=4-6(1%w/v aqueous solution)Density1. 35-1. 49g/cm3Particle size distributionStability and storage conditionsChitosan is a stable material at room temperature although it is hygroscopic subsequently drying. Chitosan should be stored in a tigjtly closed container in a cool and dry place.IncompatibilitiesChitosan is incompatible with strong oxidizing agents.SafetyChitosan is being investigated widely for use as an excipient in oral and other pharmaceutical formulations. It is also used in cosmetics. chitosan is generally regarded as biodegradable, nontoxic and non irritant material. it is biocompatible with both healthy and infected skin.ApplicationsChitosan is found profitable in many fields like sustained drug spoken communication, components of mucoadhesive social disease forms, rapid release dosage forms, improved peptide delivery, colonic drug delivery systems and use for gene delivery. Chitosan is processed into several pharmaceutical forms including gels, bead, films, microspheres tablets and coatings for liposomes.PROPRANOLOL HYDROCHLORIDE(-adrenergic blocking agents)Adrenergic nonselective -receptor antagonist. (antihypertensive, antianginal and antiarrhythmic. )STRUCTUREChemical name ()-1-isopropylamino-3-(1-naphthyloxy) propan-2-olhydrochloride molecular formula C16H21NO2. HClMolecular weight 295. 8Description A white powder, odourless and bitter in tasteSolubility Soluble Soluble 1 in 2 of water and ethanolSlightly soluble in chloroformI . PHARMACOLOGICAL ACTIONSa. Cardiovascular-Propranolol diminishes cardiac output, heart ra te, and force of compression. These effects are useful in the treatment of angina.b. Peripheral vasoconstriction-Blockade of -receptors prevents 2-mediated vasodilation. The reduction in cardiac output leads to decreased blood pressure.c. Bronchoconstriction-Blocking 2 receptors in the lungs of susceptible patients causes con brochureion of the bronchiolar smooth muscle. -blockers are thus contradicted in patients with asthma.d. increased Na+ retention- trim back blood pressure causes a decrease in renal perfusion, resulting in an increase in Na+ and plasma volume. in some cases this compensatory response tends to elevate the BP. For these patients, -blockers are often combined with a diuretic to prevent Na+ retention.II. THERAPEUTIC personal effectsa. Hypertension-propranolol discredits BP in hypertension by decreasing cardiac output.b. glaucoma-propranolo is effective in diminishing intraocular pressure in glaucoma.c. hemicrania-propranolol is also effective in reducing migrain e episodes by blocking the catecholamine induced vasodilation in the brain vasculature.d. angina pectoris-propranolol decreases the oxygen requirement of heart muscle and therefore effective in reducing the dresser pain in angina.e. myocardial infarction-propranolol and other -blockers have a protective effect on the myocardium. thus, patient who have had one myocardial infarction reckon to be protected against a second heart attack by prophylactic use of -blockers.III. ADVERSE EFFECTSa. broncho constriction-when propranolol is administered to an asthmatic patient, an immediate contraction of the bronchiolar smooth muscle prevents air from entering the lungs. Therefore, propranolol must(prenominal) never be used in treating any individual with obstructive pulmonary disease.b. arrhythmias-treatment with the -blockers must never be stopped quickly because of the risk of precipitating cardiac arrhythmias.c. disturbances in metabolism- bloackade leads to decreased glycogenolysis and decreased glucagon secretion.d. drug interaction-drugs that interfere with the metabolism of propranolol, such as cimetidine, furosemide and chlorpromazine may potentiate its antihypertensive effects. conversely those that stimulate is metabolism, such as barbiturates, phenytoin and rifampicin can mitigate its effects.PHARMACOKINETICSPropranolol is well absorbed after oral administration but has low bioavailability due to high first gear pass metabolism in liver. it is highly bound to plasma proteins.Metabolism of propranolol is pendent on hepatic blood return.DOSEOral 10mg BD to 10mg QID (average 40-60mg/day)I. V 2-8mg injected over 10min with with constant monitoring. it is not injected S. C or I. M because of irritant property.MATERIALS score OF THE MATERIALSNAME OF THE COMPANYPropranolol hydrochlorideSodium alginate ARHi-Media biosciences Ltd, Mumbai.Calcium chloride ARS. D Fine chemicals Ltd, MumbaiBarium chloride ARQualigens Fine Chemicals Ltd, MumbaiChitosan ARFluca Bio chemicals Ltd, Switzerland. (Viscosity 200-400 mPa. s)Acetic acidEQUIPMENTS USEDName of equipmentName of companyUV/Vis SpectrophotometerJASCO V-530IR SpectrophotometerJasco-FT-IR 8201 PCDifferential scanning calorimeterDSC-60 (Shimadzu, Tokyo, Japan)Optical Microscope and Stage MicrometerErma. JapanScanning Electron MicroscopeJSM 6400x-ray diffractrometerBruker AXS D8 waste apparatusElectrolab TDT-08L, USP XXIV Type I Apparatus. ChennaiRemi Hi-speed motorUniversal motors. MumbaiDigital balanceDenver Instruments18002098899 simiINTRODUCTIONMICROENCAPSULATIONA process in which very beautiful coatings of polymeric materials are deposited around particles of cheerings or droplets of liquid.Different terms for solid particle systems are employed in drug delivery among them pellets, beads, microcapsules, microspheres, millispheres are few. The terminologies of most relevant multiparticulate systems are provided here.Pellets can be defined as Small, free flowing world-wide particles manu factured by agglomeration of fine powders or granules of drug substances and excipients victimization appropriate processing equipment. The size of these particles rae usually between 0. 5 and 1. 5mm. sphericity and intra granular porosity are the two important quality attributes of pellets. The terms spherical granules and beads have been applied interchangeably to pellet system.Microspheres are solids approximately spherical particles ranging in size from 1 to 1000m. They are made of polymeric, waxy, or other protective materials, that are biodegradable synthetic polymers and modified natural products such as gums, proteins, waxes etc.Microsphere the enbtrapped substance is dispersed throughout the microsphere matrix.Microcapsule the entrapped substance is completely surrounded by distinct capsule wall.The similiarities between microsphers and microcapsules are clear and illustrations of these particles are shown in FigEncapsulation methodsTwo major classes of encapsulation metho ds have evolved, viz chemical and physical. The first class of encapsulation involves polymerisation during the process of preparing the microcapsules. physical exertions of this class are usually cognize by the name of interfacial polymerisation or in situ polymerisation. The second graphic symbol involves controlled precipitation of a polymeric solution where in physical changes usually occur.The precipitation and or gelation listed in table cover many techniques. one example isthe precipitation of water soluble polymers such as gelatin with water miscible solvents such as isopropranol. other examples include the precipitation of ethyl cellulose from cyclohexane agin by cooling, and gelation of sodium alginate with aqueous calcium salt solutions. in all cases the objective is to precipitate a per organise polymer around the core (sometimes a multi-particulate) to cause encapsulation.Process finis materialSuspended mediumInterfacial polymerizationWater soluble and insoluble mono mersAqueous/organic solventsComplex coacervationWater soluble polyelectrolyteWater wide-eyed coacervationHydrophobic polymersOrganic solventsThermal denaturationProteinsOrganic solventsSalting outWater-soluble polymerWaterSolvent evaporationHydrophilic or hydrophobic polymerOrganic or WaterHot meltHydrophilic or hydrophobic polymerAqueous/organic solventsSolvent removalHydrophilic or hydrophobic polymerOrganic solvents spraying dryingHydrophilic or hydrophobic polymerAir, nitrogenPhase separationHydrophilic or hydrophobic polymerAqueous/organic solventsPOLYMER BASED DRUG DELIVERY transcriptionThere has been growing interest in polymer based bioadhesive drug delivery systems. one of the goals of such systems is to prolong the residence time of a drug carrier wave in the Gastro Intestinal tract(GIT). The bioadhesive bond can be of a covalent, electrostatic, hydrophobicor hydrogen bond nature. ionic polymers are reported to be more adhesive than neutral polymers, and an increased cha rge density will also give better adhesion suggesting that the electrostatic interactions are of great importance. except for the oesophagus, the entire GI tract including the stomach is covered with a continous form of insoluble mucus gel. The mucus gel mainly consists of glycolproteins and due to their content of ester sulphate and sialic acid groups, the mucus layer has an overall strong net negative charge. The mucus layer has been considered as a possible site for bioadhesion and drug delivery by several groups. raw(a) polymersRecently, the use of natural polymers in the design of drug delivery formulation has received much attention due to their excellent biocompatibility, biodegradability, non toxicity and easy in availability.Polymers as carriers used in drug delivery systemThe different types of polymers for extended release preparations are given below.Biodegradable polymersThe biodegradable polymers comprised of monomers standoffed to one another through functional grou ps and have unstable linkages in the backbone. They are biologically degraded or eroded by enzymes or generated by surviving cells.NaturalAlbumin, alginate, collagen, starch, chitosan, dextran, casein, gelatine, fibrinogen etc.SyntheticPolyalklyl-cyanoacrylate, poly ethyl cyano acrylate, poly amino acids, poly amides, poly acryl amides etc.Aliphatic polyestersPoly(maleicacid), poly (glycolic acid), poly( hydroxyl group butyrate), poly (lactic acid), poly vinyl alcohol(PVA) etc.Non-biodegradable polymersPoly ethylene vinyl acetate(EVA), poly ether urethane(PEU), cellulose acetate, poly vinyl chloride(PVC), ethyl cellulose etc.In recent years a lrge number of biodegradable polymers have been investigated for their potential use as drug delivery systems. among them, sodium alginate and chitosan are very promising and have been widely exploited in pharmaceutical industry for sustained drug release. polysaccharides such as alginic acid, agar, chitin and chitosan have been used to good deal drugs for controlled drug delivery systems.Chitosan is a anaturally occurring polysaccharide comprosing of glucosamine and N-Acetyl glucosamine with unique poly cation characteristics. The polycationic nature of chitosan leads to a strong interaction with negatively charged alginate. when alginate is dropped into chitosan solution, the electrostatic interaction of carboxylic groups of alginate with the amino groups of chitosan results in the formation of a membarane on the surface of sodium alginate and improves the stability and drug content. This process has been widely used in the preparation of alginate chitosan membaranes with a solid calcium-alginate gel core. There are many advantages of the chitosan coating, such as the improvement of drug loading and bioadhesive property, as well as the prolonged drug release properties etc.Alginate(ionic, hydrophilic polymer) is a negatively charged polysachharide with high charge density and has been reported to be bioadhesive. among polyanionic polymers, alginate has been widely studied and applied for its possibility to influence the release according to the properties of its carboxyl groups as well as its biodegradability and absence of its toxicity. alginate is a naturally derived anionic polysaccharide mainly from algae belonging to the family of phaeophyceae. Alginic acid is an algal polysaccharide and a species of poly carboxylic acid. alginate consists of two sugar moieties -D mannuronic acid and -L guluronic acid which exist either in blocks or random sequences and their relative proportions determines the biofunctional properties of alginc acid. alginate is known to form complexes with divalent cations, such as Ca2+, Ba2+, and Sr2+ in aqueous solution. depending upon the composition of two sugar residues and sequential distribution in spite of appearance the molecules, the complexes form either precipitates or hydrogels. guluronic acid blocks are known to form a rigid buckled complex body part, the so called egg box array, in which chelating calcium ions are nestled in the aqueous environment of the ordered gel structure due to the spatial arrangements of guluronic block oxygen atoms of carboxyl and hydroxyl groups.Alginate has been widely used as food additive, a tablet disintegrator or gelation agent, and the mechanism of its gelation have been extensively investigated. when an aqueous solution of sodium alginate(SA) is added dropwise to an aqueous solution of calcium chloride, spherical alginate beads with regular exercise and size are liftd, since an insoluble calcium alginate matrix is formed by the cation exchange between sodium and calcium ions. alginates are known to form reticulated structure when in contact with calcium chloride ions and this characteristic has been used to produce SR particulate systems for a variety of drugs.GEL FORMATION (GENERAL MECHANISM)A gel in classical colloidal terminology, is defined as a system which owes its characteristic properties to a cross linked network of polymeric chains which form at the gel point. a considerable amount of research has been carried out in recent years to elucidate the nature of the crosslinks and determine the structure of alginate gels. alginate beads can be fain by extruding a solution of sodium alginate containing the desired drug or protein, as droplets, into a divalent crosslinking solution such as Ca2+, Ba2+, and Sr2+ . monovalent cations do not induce gelation while Ba2+, and Sr2+ ions produce stronger alginate gels than Ca2+. other divalent cations such as Pb2+, Cu2+, Cd2+, CO2+, Ni2+, Zn2+, Mn2+ will also cross link alginate gels but their use is limited due to their toxicity. The gelation and cross linking of the polymers are mainly achieved by the exchange of divalent cations and stacking of these guluronic acids with the divalent cations, and the stacking of these guluronic groups to form the characteristic egg-box structure shown in figLARGE BEAD PREPARATIONIn general, bea ds greater than 1. 0mm in diameter which can be produced by utilize a syringe, with a needle or a pipette. sodium alginate solution that contains the solubilised drug or protein is transferred dropwise into a gently agitated divalent cross linking solution. The diameter of the beads formed is dependant on the size of the needle used and the viscosity of the alginate solution . a larger diameter needle and higher viscosity solutions will produce larger diameter beads. The viscosity of SA can also influence the shape of the microbeads produced. The beads become more spherical as the concentration of SA increased. however, in general SA solutions of greater than 5% are difficult to prepare.Since, gelation occurs in an aqueous environment, alginate is a promising material as a food additive, drug formulation and useful even for encapsulation of accompaniment cells to protect them from immune responses. utilizing this stable complex formation with divalent cations, alginate gels have b een utilized for investigation of cells are considered to be the ultimate system for the pulsatile release of biologically active compounds.Formulation of delivery devices for protein and peptide drugs under aqueous conditions are desirable to avoid the undesirable decrease of bioactivities which may occur when using organic solvents or heat during formulations. since relatively stable alginate gels can be formed in aqueous environments through chelation or complexation, which are promising delivery of matrices for bioactive compounds.It has been suggested that the crosslinks were caused either by ionic bridging of 2 carboxyl groups on adjacent polymer chains via calcium ions or by chelation of single calcium ions by hydroxyl and carboxyl groups on each of a pair of polymer chains. although these bonds may play a role in the gelation mechanism which are not sufficiently energetically favourable to account for the gelation of alginate. it has been shown on the derriere of fibre diffr action data and model-building calculations that the shape of both poly-mannuronic acid segments and the polygulutended, and that these extended ribbons can stack together in sheets. on the basis of these data and the properties of gels it has been suggested that the cooperative association of either polymannuronic acid segments or polyguluronic acid segments are involved in the formation of the crosslinked network of polymer chain.This technique has shown attractive applications in different fields, including cell immobilisation, owing to its mild operating conditions. as the encapsulation method is mild, and through at room temperature in aqueous medium, several sensitive drugs, proteins, living cells, enzymes, spermatozoa etc have been successfully encapsulated through alginate beads.The primary structure of alginate depends on the producing species and for the marine species, seasonal and geographical changes might result in variations in alginates extracted from the equivalent species. The polymer is nown to form a physical gel by hydrogen bonding at low pH(acid gel)and by ionic interactions with polyvalent cations such as calcium, the cation acting as a cross linker between the polymer chains. The viscosity and primary structure of polymer are important features determining it increaseing and gelling properties.At neutral pH, sodium alginate is soluble and hydrates to form viscous solutions, but below pH3, alginic acid, water swellable but insoluble, which is rapidly formed. since the hydration characteristics of the polymer and the subsequent physical properties of the hydrated gel layer may critically influence drug release.When CA beads are treated with 0. 1M HCl, alginate gels hydrolysed to lower molecular weight fractions of alginic acid. due to conversion of COO- groups into unionised carboxylic groups, the electrostatic attraction between Ca2+ ions and COO- ions in the egg-box junction almost disappears. moreover, there may occur in ion-exchange between H+ ion(presence in the external HCl solution) and free Ca2+ ions inside the beads. thus a reduced Ca2+ ions concentration within the beads results in a weaker Ca2+ cross linked beads when put in phosphate yield at pH 6. 8. Therefore, the acid-treated beads are generally crosslinked structure more soluble alginate as constituent. when such beads are put in the phosphate buffer pH6. 8, the beads swell at a faster rate but do not attain a higher water uptake value due to loosely bound structure of the beads which is unable to retain large amount of water within the beads. moreover, there is possibility of ion-exchange between H+ ions produced due to ionization of carboxylic groups in the buffer at pH.A group of scientists developed a method of enclosing viable cells, tissues, and other labile biological substances within a semipermeable membrane. preliminary in-vitro studies of several types of microencapsulated cells and tissues(redblood cells, sperm cells, hepatica cells, hepatocytes, pancreatic endocrine tissues, and islets) were described by them. essentially, the process involves suspending the living cells or tissues in sodium alginate solution. The cell or tissue suspension is extruded through a device producing micro-droplets which fall into a calcium chloride solution and form gelled microbeads with the cells or tissues entrapped. These cell containing gel microbeads are next treated with polysine which displaces the surface layer of calcium ions and forms a permanent polysalt shell or membrane. finally, the interior calcium alginate is liquefied, either to stay in or to cum out(depending on molecular weight and size of the starting alginate) of the capsule with a calcium sequestrant such as buffered citrate solution.Gohel et al ., prepared diclofenac sodium microspeheres by using sodium alginate as a polymer and CaCl2 as a cross linking agent. in this investigation stirring speed, concentration of crosslinking agent and heavy liquid paraffin were studied, on the time required for 80% of drug dissolution. a statistical model with significant interaction terms was derived to predict t80 and drug was released by diffusion of anomalous type. The results of multiple regression analysis and F value statistics revealed that, obtaining of controlled drug release and microspheres were to be prepared using relatively lower stirring speed.Literature reports indicate wide spread use of sodium alginate for achieving sustained release of drugs, targeting gastric mucosa and increasing the bioavailability of drugs because of sodium alginates ability to form a stable and bioadhesive gel with calcium ions.Alginate also has several unique properties that have enabled it to be used as a matrix for the entrapment or delivery of a variety of proteins, macromolecules and cells.USES Of Alginate BeadsA relatively inert aqueous environment within the matrix.A mild room temperature encapsulation process free of organic solventA high gel porosity which allows for high diffusion rate of macromoleculesThe ability to control this porosity with simple coating procedures.Dissolution and biodegradation of the system under normal physiological conditions.Standard graph for propranolol hydrochlorideA stock solution of propranolol hydrochloride was prepared by dissolving 100mg of the drug in 100ml of the phosphate buffer of pH6. 8 to give 1mg/ml solution. ten millilitres of stock solution was diluted to 100ml using phosphate buffer f pH6. 8 to produce 100g/ml working stock solution. from this working solution, dilutions were made with phosphate buffer of pH6. 8 to produce 10, 20, 30, 40 and 50 g/ml. The max of the drug was unyielding by scanning the dilutions between 400 and 200nm using a Shimadzu 1400 UV visible spectrophotometer. At this wavelength, the absorbances of all the other solutions were measured against a blank. Standard writhe between concentration and absorbance was plotted.COMPATIBILITY STUDIESOne of the requiremen ts for the selection of suitable polymers or carriers for pharmaceutical formulation is its compatibility. Therefore in the present work a compatibility study was done by using Infra Red spectroscopy (IR) and Differential Scanning Calorimetry (DSC) to find out if there is any possible chemical interaction between propranolol hydrochloride and the polymers.DIFFERENTIAL SCANNING CALORIMETRY (DSC)Differential Scanning calorimetric analysis was used to characterize the caloric behaviour of the drug substances. It was performed by using DSC-60(Shimadzu, Tokyo, Japan) calorimeter to study the thermal behaviour of selected formulations. The instrument comprised of calorimeter (DSC60), flow controller (FCL60), thermal analyzer (TA60) and operating software(TA 60). The samples were heated in hermetically sealed aluminium pans under nitrogen flow (30ml/min)at a scanning rate of 5C/min from 24 + 1C to 300C. An empty aluminium pan, sealed in the same way as the sample was used as a reference.S CANNING ELECTRON MICROSCOPYScanning electron microscopy is used to obtain the surface topographical flick of beads. SEM photographs of prepared formulations were taken with (Instrument JSM-6390)at different magnification ranging from 30 to 5000x at room temperature. The samples were mounted on double sided adhesive tape that has previously been secured on bruiser stubs. The acceleratio